CPE細胞病變(CPE)
3)microdose cytopathogenic efficiency (CPE) assay微量細胞病變(CPE)法
4)CPE細胞病變效應法
1.Methods CPE observation and plaque reduction assay were used to test the anti-HSV-1 effect of Ilex Kudmcha C.方法采用細胞病變效應法(Cytopatho-genic effect,CPE)和空斑減數實驗(Plaque reduction assay)測定不同苦丁茶提取物抗HSV-1活性,計算藥物對病變的抑制率和半數抑制濃度(50%inhibiting concentration,IC50),并從藥物對細胞的保護,對病毒增殖的影響及對病毒感染細胞的綜合作用3個方面初步探索苦丁茶提取物抗HSV-1病毒活性的機理。
5)cytopathic effect細胞病變效應
1.Objective:To evaluate the difference between 5-diphenyl tetrazolium bromide(MTT)and cytopathic effect(CPE)in screening the antiviral medicine.目的:比較四甲基偶氮唑鹽(MTT)法與細胞病變效應(CPE)在篩選抗病毒藥物中的異同。
2.We observed the cytotoxicity of Qingfei quyu decoction on A549 cells by using MTT method;after that,observed the protective effect for the cytopathic effect of A549 cells by Qingfei quyu decoction below media實驗部分:清肺祛瘀湯抗RSV的體外實驗,觀察清肺祛瘀湯對A549細胞毒性,用MTT法測定藥物的細胞毒性;再以半數毒性濃度下的清肺祛瘀湯,觀察其對A549的細胞病變效應(CPE)的保護作用,以了解藥物的體外抗病毒作用。
3.By using cytopathic effect (CPE) as the index, the antivirus effects in vitro and cytotoxicity of five Chinese drug preparations were investigated.方法建立流感病毒感染MDCK細胞、柯薩奇病毒和呼吸道合胞病毒感染VeroE6細胞的模型,以細胞病變效應(CPE)為觀察指標,了解不同濃度5種中藥制劑體外抗病毒效果及細胞毒性。
6)CPE細胞病變效應
1.Methods The cell killing effect was observed by CPE(cytopathic effect) assay;the cell growth inhibition and virus propagation efficiency was measured by MTT assay and plaque formation assay,respectively.方法:通過細胞病變效應(CPE)觀察d l1520對肝癌細胞株的殺傷作用;MTT法測定對肝癌細胞生長抑制作用,并通過空斑法測定病毒增殖,探討其殺傷機理。
延伸閱讀
效應細胞 效應細胞 在免疫應答效應階段發揮作用的細胞。例如產生抗體的效應細胞主要是漿細胞,發揮同種移植反應的效應細胞是T淋巴細胞。
