1)Glycine-SDS-PAGE甘氨酸-SDS-PAGE
1.The traditionally used Glycine-SDS-PAGE method had some shortcomings in resolving proteins with molecular masses below 15 Ku while the classical Tricine-SDS-PAGE method was more effective for separating proteins with low molecular weights, but ineffective for proteins with molecular masses more than 60 Ku.結果表明,通常使用的甘氨酸-SDS-PAGE法對B-乳清中低分子量蛋白分離效果差,Tricine-SDS-PAGE三層膠系統分離低分子量蛋白有優勢,但分離60Ku附近的蛋白效果不好;16。
2)SDS PAGESDS-PAGE
1.And their viral structure proteins were analyzed by SDS PAGE.以 La Sota毒株和分離到的 4株分別來源于信鴿、肉鴿和雞的新城疫毒株為研究對象 ,設計了 3對引物用反轉錄 -聚合酶鏈反應 (RT-PCR)對它們的融合蛋白基因 (F)進行分段擴增 ,并且采用 SDS-PAGE進行結構蛋白分析。
2.Two materials of GN21 and P38 were analysed by means of SDS PAGE and RAPD.利用 SDS-PAGE蛋白質電泳及 RAPD技術對貴農 2 1 ( GN2 1 )和 P3 8兩個材料進行分析 ,發現G2 1、P3 8和它們的親本的 SDS-PAGE蛋白質圖譜表現出一些差異。
3.After that the mitochondrions polypeptides and the dissolved proteins were abstracted and analyzed by SDS PAGE.用普通小麥的不育系和保持系為材料 ,經高溫處理以后 ,提取其線粒體多肽和可溶性蛋白做 SDS-PAGE分析 ,發現不育系與保持系在熱激后其線粒體多肽的電泳圖譜均有相互趨同的現象 。
英文短句/例句
1.Characters of Protein Migrating Towards Cathode in SDS-PAGE;SDS-PAGE中向負極泳動的蛋白的性質
2.Detection of Heating Endpoint Temperature for Fish by SDS-PAGE MethodSDS-PAGE電泳法檢測魚肉熱處理終點溫度
3.SDS-PAGE Analysis of Secalin Variations in Secale黑麥屬貯藏蛋白的SDS-PAGE分析
4.Compare of the SDS-PAGE spectrum of the components of soy protein before and behind gelatification大豆蛋白凝膠前后SDS-PAGE圖譜比較分析
5.An Experimental Instruction of SDS-PAGE Suitable to Educational Activities一種適用于教學的SDS-PAGE電泳實驗指導
6.Analysis of Oats Glutenin with SDS Polyacrylamide Gel Electrophoresis (SDS-PAGE)燕麥麥谷蛋白SDS-PAGE電泳分析
7.Purification and partial characterization of turbot (Scophthatmus maximus) vitellogenin大菱鲆卵黃原蛋白的分離純化及Native-PAGE、SDS-PAGE性質鑒定
8.Diversity of SDS-PAGE Phenotypes of Glycinin in Seeds of Soybean Germplasm Resources大豆種質資源種子11S球蛋白的SDS-PAGE表型多樣性
9.Identification of the Protein Composition of Five Different Feedstuffs with Tricine-SDS-PAGE SystemTricine-SDS-PAGE鑒定不同品質飼料蛋白質的組成
10.Leaf Protein Extraction Methods Suitable for SDS-PAGE Analysis in Apple適于SDS-PAGE分析的蘋果葉片蛋白質提取方法
11.The image analysis of pleural fluid proteins in lung adenocarcinoma and lung tuberculosis separated by SDS-PAGE肺結核性及腺癌性胸水SDS-PAGE蛋白圖譜分析
12.Quantification of Individual High and Low Molecular Weight Glutenin Subunits of One Wheat Kernel Using SDS-PAGE and Scanning Densitometry;用SDS-PAGE和密度掃描定量檢測小麥單籽粒高、低分子量麥谷蛋白亞基
13.Studies on the Different Expression of Protein in Chicken, Quail, Chicken-quail Hybrid during the Early Embryo by SDS-PAGE;利用SDS-PAGE分析雞、鵪鶉及其屬間雜交種早期胚胎蛋白表達的差異
14.Study on the Purity Identification of Hybrid Rice Seeds by Salt-soluble Protein SDS-PAGE;應用鹽溶蛋白SDS-PAGE技術鑒定雜交水稻種子純度的研究
15.Polyelectrolyte as Vehicles for Isolation and Purification of Alkalescence Protein and the Conditions Optimization of Alkalescence Peptides SDS-PAGE;高分子電解質分離堿性蛋白的方法及堿性多肽SDS-PAGE條件的優化
16.Diversity of SDS-PAGE Phenotypes of Kafirins in Seeds of Sorghum Germplasm Resources高粱種質資源種子醇溶谷蛋白的SDS-PAGE表型多樣性
17.Verification of Nodulation Ability of Strains Isolated from Mimosa spp. Nodules Using Whole Cell Protein SDS-PAGE Molecular Marker Method應用全細胞蛋白SDS-PAGE分子標記技術驗證含羞草根瘤菌的結瘤能力
18.Comparative analysis of muscle larval antigens of Trichinella spiralis and Trichinella pseudospiralis by SDS-PAGE and two-dimensional gel electrophoresis旋毛蟲與偽旋毛蟲肌幼蟲抗原的SDS-PAGE和雙向電泳分析
相關短句/例句
SDS PAGESDS-PAGE
1.And their viral structure proteins were analyzed by SDS PAGE.以 La Sota毒株和分離到的 4株分別來源于信鴿、肉鴿和雞的新城疫毒株為研究對象 ,設計了 3對引物用反轉錄 -聚合酶鏈反應 (RT-PCR)對它們的融合蛋白基因 (F)進行分段擴增 ,并且采用 SDS-PAGE進行結構蛋白分析。
2.Two materials of GN21 and P38 were analysed by means of SDS PAGE and RAPD.利用 SDS-PAGE蛋白質電泳及 RAPD技術對貴農 2 1 ( GN2 1 )和 P3 8兩個材料進行分析 ,發現G2 1、P3 8和它們的親本的 SDS-PAGE蛋白質圖譜表現出一些差異。
3.After that the mitochondrions polypeptides and the dissolved proteins were abstracted and analyzed by SDS PAGE.用普通小麥的不育系和保持系為材料 ,經高溫處理以后 ,提取其線粒體多肽和可溶性蛋白做 SDS-PAGE分析 ,發現不育系與保持系在熱激后其線粒體多肽的電泳圖譜均有相互趨同的現象 。
3)SDS-PAGE electrophoresisSDS-PAGE電泳
1.A method of SDS-PAGE electrophoresis was used to identify genuineness of seed and cultivar purity of malting barley.采用操作簡便、分辨率高的種子醇溶蛋白SDS-PAGE電泳技術對常用澳大利亞和法國啤酒大麥進行鑒定,利用Gel-Pro軟件對電泳圖譜進行條帶分析和比較,建立了澳大利亞和法國啤酒大麥品種標準圖譜庫。
2.A method of SDS-PAGE electrophoresis was developed to identify genuineness of seed and cultivar purity of malting barley.開發了操作簡便、電泳圖譜清晰、分辨率高的啤酒大麥種子醇溶蛋白SDS-PAGE電泳方法,利用Cel-Pro軟件對電泳圖譜進行條帶分析和比較,建立了每個品種的蛋白質“指紋”,組成加拿大啤酒大麥品種標準圖譜庫。
3.The effect of different alcohol content in protein extraction on the molecular weight of soybean protein concentrate was systematically studied by using SDS-PAGE electrophoresis and by examining protein dissolution rate.對浸提液采用SDS-PAGE電泳法測定蛋白質分子量,并對蛋白質溶出率進行測定,探索不同醇濃度對浸提液中蛋白溶出率及分子量的影響,為醇法制取大豆濃縮蛋白浸提液再加工、回收蛋白提供了一定的理論基礎。
4)SDS-substrate-PAGESDS-底物-PAGE
1.SDS-substrate-PAGE further showed that there were four sorts of alkaline proteases in the crude extract,in which three sorts were serine proteases(trypsins).SDS-底物-PAGE電泳表明,草魚腸道粗酶中有4種堿性蛋白酶,其中3種是絲氨酸蛋白酶(胰蛋白酶),1種是非絲氨酸蛋白酶,它們的相對分子質量分別為:26 400、30 750、43 000、約105 000。
2.SDS-substrate-PAGE method was modified and used to characterize the proteinases from the digestive organs of freshwater fish.改進SDS-底物-PAGE技術以用于分析檢測淡水魚中的消化蛋白酶。
5)SDS-PAGESDS-PAGE電泳
1.Study on the Thermal Stability of Ginseng Protein by SDS-PAGE and Gel Filtration Chromatography;SDS-PAGE電泳和高效凝膠過濾色譜法研究人參蛋白熱穩定性
2.SDS-PAGE Analysis of Soluble Proteins from Taenia saginata;牛帶絳蟲可溶性抗原SDS-PAGE電泳初步分析
3.[Method] The purity and immune activity of canine serum IgG were identified by SDS-PAGE electrophoresis and western blot after extracted and purified by ammonium sulfate salting-out method combined with SephadexG-200 gel filtration chromatography.[方法]采用硫酸銨鹽析法結合SephadexG-200凝膠柱層析提取純化犬血清IgG,利用SDS-PAGE電泳和免疫印跡法鑒定所得產品的純度和免疫活性。
6)sodium dodecylsulphate-polyacrylamide gel electrophoresisSDS-PAGE法
1.Methods Gel filtration chromatography(GFC),sodium dodecylsulphate-polyacrylamide gel electrophoresis(SDS-PAGE) technique and matrix-assisted laser desorption/ionizationtime of flight mass spectrometry(MALDI-TOF-MS)were used to determine the relative molecular weight,respectively.方法采用凝膠過濾層析法、SDS-PAGE法和基質輔助激光解吸附電離飛行時間質譜法(MALDI-TOF)三種方法測定一種新型蚯蚓脫氧核糖核酸酶(EWD2)的分子量。
延伸閱讀
PAGE分子式:CAS號:性質:用聚丙烯酰胺為支持基質的電泳程序。一般說來,實現聚丙烯酰胺凝膠電泳有兩種類型。(1)單向電泳:采用完整的蛋白質或用十二烷基磺酸鈉(SDS)處理的蛋白質的單向泳動,在有凝膠的平板上平行分離(過去也有用在玻管中的圓筒形棒狀凝膠進行電泳的,現已很少有人使用)。(2)雙向電泳:首先用天然蛋白質進行分離,然后凝膠平板再用SDS處理,樣品便在第二向得到分離。第一次分離了各種各樣的蛋白質,因此第二向分離的是蛋白質亞基。主要優點是:(1)合成聚合物,故重復性良好;(2)分離能力好;(3)通過增減丙烯酰胺單體和交聯劑(N,N′-亞甲基雙丙烯酰胺)的濃度,可以調節凝膠的孔徑大小;(4)操作簡便、時間短;(5)化學性質穩定、機械性能好,柔軟;(6)在酸性或堿性緩沖液中均可進行電泳,而且可加入兩性電解質進行等電點電泳,可用含電解質表面活性劑(SDS)或非電解質表面活性劑(Np40、Tritonx-100等)的凝膠進行電泳,亦可使兩者組合進行雙向電泳等等,使用范圍廣泛,利用價值日益提高;(7)由于染色技術的進步,可以進行定量,也可檢測出極微量的斑點(瓊脂糖電泳)。
