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免疫捕獲RT-PCR,IC-RT-PCR
1)IC-RT-PCR免疫捕獲RT-PCR
1.In this study,based on the previously reported viral coat protein(CP)gene sequences,specific primers for ORSV and CyMV were designed,and four methods,including ELISA,RT-PCR,nested RT-PCR and IC-RT-PCR were performed for molecular detection of the two viruses.本研究根據病毒外殼蛋白基因設計特異性引物,應用ELISA、普通RT-PCR、巢式RT-PCR和免疫捕獲RT-PCR4種方法進行了檢測研究與比較。
2)Immuno-capture nested RT-PCR免疫捕獲巢式RT-PCR
3)Multiplex Immuno-capture RT-PCR多聯免疫捕獲RT-PCR
4)one-step ic-rt-pcr免疫捕獲一步RT-PCR
英文短句/例句

1.Detection of Bean Pod Mottle Virus by One-step IC-RT-PCR菜豆莢斑駁病毒免疫捕獲一步RT-PCR檢測
2.Detection of Salmonella in milk by Combination of Immunomagnetic Separation and PCR免疫磁珠捕獲-PCR檢測牛乳中沙門氏菌的研究
3.Detection of Food-and-Mouth Disease by RT-PCR and Immune Antibody Surveillance;口蹄疫的RT-PCR檢測及免疫抗體監測
4.Development of an Antigen Capture Reverse Transcriptase/Polymerase Chain Reaction for Detection Foot-and-Mouth Disease Virus;抗原捕獲反轉錄聚合酶鏈式反應(AC-RT/PCR)檢測口蹄疫病毒方法的建立
5.Real-time Immuno-RT-PCR for Antigen Detection with Armored RNA as Reporter;一種以armored RNA作為報告基團超靈敏的實時免疫RT-PCR方法的建立
6.Immunobeads Combine RT-PCR Detection of Gastric Cancer Tumor Markers α4GnT;免疫磁珠聯合RT-PCR檢測胃癌腫瘤標志物α4GnT
7.Detection of Bean pod mottle virus in soybean by one step assay of RT-PCR and real-time fluorescent RT-PCRRT-PCR和實時熒光RT-PCR一步法檢測大豆中菜豆莢斑駁病毒
8.One-step RT-PCR for detection of SARS associated coronavirus一步法RT-PCR檢測SARS冠狀病毒技術研究
9.Detecting HCV RNA by one-step short-product RT-PCR一步短片段RT-PCR方法檢測hcv RNA
10.A Study on the one-step RT-PCR for detection of potato Viruses;馬鈴薯病毒一步法RT-PCR診斷研究
11.Detection of Peripheral Blood Micrometastasis in Patients with Gastric Carcinoma by Combinative Use of Negative and Positive Immunomagnetic Beads with Quantitative RT-PCR;聯用陰性和陽性免疫磁珠及定量RT-PCR檢測胃癌外周血微轉移
12.Establishment of RT-Nested PCR Detection Method about Classical Swine Fever Virus and Study on Immunity and Eradication of CSF in Breeding Swine Field豬瘟病毒套式RT-PCR檢測方法的建立和種豬場豬瘟免疫與凈化研究
13.Detection of sentinel lymph node micrometastases in cervical cancer patients with RT-PCR and immunohistochemistryRT-PCR與免疫組化法檢測宮頸癌前哨淋巴結微轉移的探討
14.Application of immunomagnetic separation in combination with fluorescence quantitative RT-PCR for detecting micrometastasis in circulation in patients with prostate cancer免疫磁珠分選聯用實時FQ RT-PCR檢測前列腺癌患者外周血微轉移
15.Immunocapture-Universal Primer PCR Detection of Salmonella sp. in Foods免疫捕捉-通用引物PCR檢測食品中沙門氏菌
16.One-step Duplex RT-PCR Assay for Detection SARS Associated Coronavirus一步法雙重RT-PCR檢測SARS冠狀病毒技術研究
17.Establishment of One-step RT-PCR Detection Method of Live Hepatitis A VirusRT-PCR一步法檢測甲型肝炎活病毒方法的建立
18.Detection of the genomic sequences of two alphaviruses by one-step RT-PCR兩種甲病毒基因組序列的一步RT-PCR檢測
相關短句/例句

Immuno-capture nested RT-PCR免疫捕獲巢式RT-PCR
3)Multiplex Immuno-capture RT-PCR多聯免疫捕獲RT-PCR
4)one-step ic-rt-pcr免疫捕獲一步RT-PCR
5)Immunocapture Reverse transcription a免疫捕獲反轉錄聚合酶鏈式反應(IC-RT-PCR)
6)immunocapture reverse transcription PCR免疫捕獲反轉錄PCR
1.The samples were then analyzed by immunocapture reverse transcription PCR (IC RT PCR) with primers specific for SCMV, Maize dwarf mosaic virus (MDMV), Sorghum mosaic virus (SrMV), and Johnsongrass mosaic virus (JGMV), respectively.利用甘蔗花葉病毒 (Sugarcanemosaicvirus,SCMV)單克隆抗體細胞株 2B5腹水和SCMV、玉米矮花葉病毒 (Maizedwarfmosaicvirus,MDMV)、高粱花葉病毒 (Sorghummosaicvirus,SrMV)和約翰遜草花葉病毒 (Johnsongrassmosaicvirus,JGMV)的特異性引物對我國浙江、江蘇、上海、山東、河南、河北、北京、山西、陜西、甘肅、四川、云南 12省市 15個地點的 176株玉米矮花葉病病樣分別進行了間接ELISA和免疫捕獲反轉錄PCR(IC RT PCR)檢測 ,結果表明這些病樣均含有SCMV ,而無MDMV、SrMV或JGMV存在 ,表明上述 12省市的玉米矮花葉病病原為SCMV。
延伸閱讀

免疫PCR分子式:CAS號:性質: 通過應用一個對DNA和抗體具雙重結合活性的接連分子使作為標志物的DNA分子特異地結合到抗原-抗體復合物上,從而形成一種特異性抗原-抗體-DNA復合物。附著的DNA標志物可用適當的引物進行PCR擴增。特異性PCR產物的存在證明DNA標志物分子特異性地附著于抗原-抗體復合物上,進而證明有抗原存在。目前最為敏感的檢測方法,理論上可測得一個抗原分子的存在。
韩国伦理电影